Obtaining a graduate degree would be so much easier if the analytical instrumentation would just work…For those of you would don’t have to run various chromatography instruments (ICs, HPLCs, GCs), thermo-cyclers, spectrophotometers, or any of the other numerous finicky pieces of laboratory equipment, I envy you. You haven’t had to start your day thinking you would be able to run 100+ samples and get another figure for your thesis, only to spend not just a day but a whole week troubleshooting a mysterious problem, eventually determining you’ll have to order a part that will be delivered in three more weeks just to determine the concentration of your chemical of interest. This of course holds up all the other experiments you had planned to set up. I welcome you all to the joys of basic wet science research.
When I find myself in these situations I take a deep breath and think of all the reading I’ll be able to get done while I wait. In my experience these situations usually arise from a few common problems and are a major part of the experimental process. First, make sure you really read the instrument manual before you attempt to use anything or try to fix it. Many times an instrument isn’t working because someone else, who had no idea what they were doing, decided to make a “repair.” This is one reason it is important for senior members of the lab to instruct the new lab members on proper usage. Secondly, remember to perform routine maintenance, as neglected instruments are like high maintenance boyfriends and girlfriends. They will not work solely out of spite if ignored for too long. Instruments work best when used and maintained on a regular basis. Third, always remember that this is part of the “learning” process. You never really understand how something works until you have taken it apart and put it back together a million times. Now not only are you an expert on the instrument, but you can also understand and interpret your data better since you know the limitations of the measurement. Your advisor and other graduate students will agree that this is a large part of the experimental process.
Lastly, if all else fails, blame an undergraduate and take a long weekend or a mental health day. Delays are only to be expected when relying on group used equipment and if you are lucky someone else will have fixed it by the time you get back. Plus working this hard makes obtaining the data that much sweeter. So the next time an instrument, computer, or your “favorite” piece of equipment gives you a strange error message remember that you are not alone and that this is all part of the process.